Oxygen consumption, growth and calculated food requirement of the swimming crab Portunus trituberculatus in its early developmental stage.

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Molecular cloning and characterization of prophenoloxidase gene in swimming crab Portunus trituberculatus.

Phenoloxidase (PO), a melanin-synthesizing enzyme, found as a zymogen (proPO) in hemolymp, plays an important role in arthropod defence. In this study, a prophenoloxidase (proPO) cDNA was cloned from the haemocytes of swimming crab Portunus trituberculatus by a reverse-transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA end (RACE) method. Analysis of the nucleotide ...

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Screening of genes related to ovarian development in the swimming crab, Portunus trituberculatus, by suppression subtractive hybridization.

The swimming crab, Portunus trituberculatus, is an important marine animal and is widely cultured in China. In the present study, suppression subtractive hybridization was applied to identify the differentially expressed genes in the ovaries of mature and immature P. trituberculatus. One hundred and seventy six expressed sequence tag (ESTs) were identified, of which 100 were down-regulated, and...

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Changes in oxygen consumption and heart rate of the blue swimming crab, Portunus pelagicus (Linnaeus, 1766) following exposure to sublethal concentrations of copper.

Weight specific oxygen consumption and heart rates were investigated in blue swimming crab, Portunus pelagicus to indicate the stress in response to increasing salinities (50, 75, 100 and 125%SW) and increasing copper concentration (0, 0.75, 1.5, 3 and 6 ppm copper) at temperature 25 degrees C. Oxygen consumption (micromol.g(-1).h(-1) was measured using closed system respirometer (Strathkelvin ...

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Transcriptome Analysis of the Portunus trituberculatus: De Novo Assembly, Growth-Related Gene Identification and Marker Discovery

BACKGROUND The swimming crab, Portunus trituberculatus, is an important farmed species in China, has been attracting extensive studies, which require more and more genome background knowledge. To date, the sequencing of its whole genome is unavailable and transcriptomic information is also scarce for this species. In the present study, we performed de novo transcriptome sequencing to produce a ...

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ژورنال

عنوان ژورنال: NIPPON SUISAN GAKKAISHI

سال: 1988

ISSN: 1349-998X,0021-5392

DOI: 10.2331/suisan.54.1137